Analysis Note

ControlBrain tissue, Rat Purkinje cell dendrites, rat hippocampus lysate, HEK 293 cells expressing NR2B.

Application

Anti-NMDAR2B Antibody is an antibody against NMDAR2B for use in IH, IP & WB.

Research CategoryNeuroscience

Immunohistochemistry:
1:1,000-1:2,000 dilution of a previous lot was used on immunohistochemistry.

Immunoprecipitation:
3 µL of a previous lot of this antibody (under appropriate conditions) quantitatively immunoprecipitated all NMDAR2B in 200 µg of rat brain.

Western Blot Analysis:
1:100-1:2,000 dilution of a previous lot (NMDAR2B is present in high concentrations in the hippocampus).

Optimal working dilutions must be determined by the end user.

Research Sub CategoryNeurotransmitters & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

NMDA receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). GRIN2B may be a candidate gene for the neurodegenerative disorder dentato-rubro-pallidoluysian atrophy (DRPLA).
Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage dependent Mg2+ blockade and high Ca2+ permeability. The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer′s disease and also epilepsy and ischemic neuronal cell death.

Immunogen

Epitope: C-terminus

C-Terminal Fusion Protein of the NMDAR2B (30 kDa).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

ImmunoAffinity Purified

Purified rabbit polyclonal lyophilized from ammonium bicarbonate (5 mM). Some residual salt may also be present. Reconstitute with 50 µL of PBS. Contains no preservatives.

Format: Purified

Specificity

NMDAR2B. Strong and specific immunolabeling of the 180 kDa NMDAR2B subunit of the NMDA receptor. No reactivity to NMDAR2A or NMDAR2C by Western blot. Immunolabeling has been blocked by preadsorption of the antibody with the C-terminal fusion protein that was used to generate the antibody.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Target description

180 kDa